Colchicine delays cartilage destruction after DMM in mice. (A, B) The right knees of the control, sham, DMM, and DMM + colchicine mice were collected 12 weeks post-surgery. Colchicine administration was started 5 days post surgery. Sections were subjected to Safranin O-fast green staining (A) and immunohistochemistry (B). Images are representative of control, n = 5; sham, n = 10; DMM, n = 10; DMM + colchicine, n = 10. OARSI score, osteophyte size, synovium membrane inflammation score, and subchondral bone graded are evaluated. Data represent mean with 95%CI. Data were analyzed using the one-way ANOVA with Tukey’s post-hoc test. *adjusted p < 0.05, **adjusted p < 0.01.