FIG. 3.

Deletion analysis of c-Myc protein turnover. COS-7 cells were transiently transfected with 2 μg of an expression plasmid encoding wild-type human (WT hu) c-Myc (pSV-myc) or c-Myc with the indicated deletion mutation. Forty-eight hours after transfection, cells were subjected to pulse-chase analysis as described in the legend to Fig. 1A, and c-Myc was immunoprecipitated with anti-MycFL.