REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-Myc/c-Myc Antibody (9E10) | Santa Cruz | Cat# sc-40 |
Alkaline phosphatase goat anti-mouse IgG | Jackson | Cat# 115-055-062; RRID: AB_2338533 |
NBT/BCIP stock solution | Roche | Cat# 11681451001 |
Chemicals, peptides, and recombinant proteins | ||
Tamoxifen | Sigma-Aldrich | Cat# T5648 |
sPirB | Bochner et al., 2014 | N/A |
Tetrodotoxin citrate | A.G. Scientific | Cat# T-2785 |
Picrotoxin | Tocris | Cat# 1128 |
NBQX | Tocris | Cat# 0373 |
(R)-CPP | Tocris | Cat# 0247 |
DNI-glutamate trifluoroacetate | Femtonics Ltd. | Cat# 1951 |
L-689,560 | Tocris | Cat# 0742 |
Experimental models: Organisms/strains | ||
Mouse: PirB−/− | Syken et al., 2006 | N/A |
Mouse: PirBfl/fl | Djurisic et al., 2019 | N/A |
Mouse: CaMKIIa-CreER | The Jackson Laboratory | Jax# 012362 |
Software and algorithms | ||
DeepLab Cut | Mathis et al., 2018 | N/A |
ImageJ | NIH ImageJ | RRID: SCR_003070 |
MATLAB | Mathworks | RRID: SCR_001622 |
Custom MATLAB scripts for analysis | Custom | DOI: 10.5281/zenodo.5146405 |
Other | ||
Micro-osmotic pump | Alzet | Cat# 0004317 |