A. Trabecular and medial compartment subchondral bone mineral density (BMD) was increased by a HF diet, especially in sedentary mice. B. Trabecular bone morphology was largely unaffected by a HF diet and voluntary wheel running. An exception was the trabecular connectivity density, which was reduced by a HF diet in sedentary but not exercise mice. BV=bone volume, TV=total volume. Data points are values for individual animals, and bars are mean +/− 95% CI. Significant differences between groups are indicated by bars that do not share a common letter (2-factor ANOVA, p≤0.05, followed by Fisher’s LSD post-hoc, p<0.05).