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. Author manuscript; available in PMC: 2022 Oct 21.
Published in final edited form as: Cell Chem Biol. 2021 May 19;28(10):1460–1473.e15. doi: 10.1016/j.chembiol.2021.04.024

Figure 6. Microtubule gliding assay in the presence of compound 20.

Figure 6.

(A) Schematic of microtubule gliding assay. GFP-Sc-Dyn, rhodamine-labeled microtubule (MT), coverslide (light blue) and total internal reflection fluorescence (TIRF) laser are indicated. (B) Montages of fluorescent microtubules moving on GFP-Sc-Dyn-coated glass coverslips in the presence of 1% DMSO or compounds 6 and 20 (25 μM) after 30 minute incubation. See also Movie S1. (C) Measurements were made both immediately (black) after treatment (1% DMSO: 83 ± 23 N=190, 25 μM compound 20: 35 ± 19 N=202, 25 μM compound 6: 71 ±1 9 N=149) and 30 minutes (red) after treatment (1% DMSO: 73 ± 21 N=85, 25 μM compound 20: 3 ± 5 N=155, 25 μM compound 6: 66 ± 21 N=103). The two timepoint measurements were taken in the same flow cells. Data pooled from four flow cells per condition, taken over two experimental days using two separate preparations of GFP-Sc-Dyn. Each measurement shown as a light color point, mean ± standard deviation shown in bold color.