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. 2021 Oct 11;9:737872. doi: 10.3389/fcell.2021.737872

FIGURE 4.

FIGURE 4

Irisin stimulated CXCL1 release predominantly from subcutaneous (A) and deep-neck (B) area differentiated adipocytes. Preadipocytes (Pre) were plated and differentiated into adipocytes (Ad) on Ibidi chambers, with or without irisin treatment as in Figures 1–3. Cells were treated with 100 ng/ml brefeldin-A for 24 h to block the secretion of CXCL1, which was followed by fixation and image acquisition by confocal microscopy. Propidium Iodide (PI) was used to stain the nucleus. BF represents the bright field image. Confocal images of differentiated adipocytes were shown followed by wider coverage of undifferentiated and differentiated adipocytes. Scale bars represent 10 μm for single differentiated Ad and 30 μm for wider coverage of Pre and Ad. Yellow and green arrows point the undifferentiated preadipocytes and the differentiated adipocytes, respectively. Quantification of fluorescence intensity normalized to per cell are shown on the right bar graphs. Data presented as Mean ± SD. n = 35 cells (A) and 50 cells (B) from two independent donors. ****p < 0.0001. Statistics: One-way ANOVA with Tukey’s post-test.