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. 2021 Oct 11;15:733007. doi: 10.3389/fnint.2021.733007

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Copyright © 2021 Cisternas, Gherardelli, Salazar and Inestrosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Age-associated reduction in hippocampal glucose metabolism in degus. (A) Uptake of radioactive glucose in hippocampal slices obtained from 1- to 6-year-old degus was measured over 60 min. (B) Glucose uptake at 60 min of (A). Hippocampal slices from 1-, 3-, 5-, and 6-year-old degus were analyzed for (C) glycolytic flux over 60 min. (D) Quantification of glycolytic flux at 60 min of (C). (E) The pentose phosphate pathway (PPP) was measured in hippocampal lysates of 1- to 6-year-old degus. The data represent the mean ± SEM of five independent biological replicates in three technical replicates. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 by one-way ANOVA (B,D,E) or two-way ANOVA (A,C), followed by Bonferroni's post-hoc test.