TABLE 2.
Metabolic capacity of the Ace Lake Omnitrophota inferred from metagenome-assembled genomes (MAGs).
| Omnitrophota |
Metabolic capacity |
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| All genera and species | Anaerobic heterotroph incomplete TCA cycle, terminating at fumarate (reductive branch) and 2-oxoglutarate (oxidative branch) secreted glycoside hydrolases and peptidases V-type ATP synthase | |||||
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| Candidatus Genus | Candidatus Species | MAGs (% completeness) | Fermentation substrates | Other carbon metabolism | Other bioenergetic complexes/enzymes | Hydrogenase |
| Aceula | lacicolaT | 3300035698_1100 (92%) | Sugars, glycerol | Glycogen synthesis | Rnf, Sud, HppA | NiFe hydrogenase (Group 3d) |
| meridiana | 3300035698_985 (92%) | |||||
| Zapsychrus | exili T | 3300035698_1555 (89%) | Sugars, glycerol | Glycogen synthesis | Rnf, Nqr, Sud, HppA | NiFe hydrogenase (Group 3d) |
| Gygaella | obscura T | 3300035698_1934 (85%) | Sugars, 2-oxoacids | Glycogen synthesis | Rnf, Sud, HppA | FeFe hydrogenase (Group A3) |
| Susulua | stagnicola T | 3300035698_1005 (82%) | Sugars, 2-oxoacids | Glycogen synthesis | Rnf, Sud, HppA | FeFe hydrogenase (Group A3) |
| Saelkia | tenebricola T | 3300035698_749 (91%) | Sugars, alcohols | Glycogen synthesis | Rnf, Mrp, Sud, HppA | NiFe hydrogenase (Group 4g), Mrp-linked |
| Kaelpia | aquatica T | 3300035698_2000 (93%) | Sugars, 2-oxoacids, alcohols | Glycogen synthesis | Rnf, Sud, HppA | FeFe hydrogenase (Group A3) |
| imicola | 3300035698_1655 (92%) | |||||
| Kappaea | frigidicola T | 3300035698_1500 (76%) | Sugars | Trehalose synthesis | Rnf, HppA | FeFe hydrogenase (Group A3) |
| Tantalella | remota T | 3300035698_1097 (93%) | Sugars, glycerol, 2-oxoacids, alcohols | Glycogen synthesis, trehalose synthesis | Rnf, Sud, HppA | NiFe hydrogenase (Group 3b)/sulfhydrogenase |
| Aadella | gelida T | 3300035698_848 (91%) | Sugars, glycerol, 2-oxoacids | Trehalose synthesis | Rnf, Sud | NiFe hydrogenase (Group 3b)/sulfhydrogenase |
| Gorgyraea | atricola T | 3300035698_32 (93%) | Sugars, 2-oxoacids | Wood–Ljungdahl pathway, glycogen synthesis, trehalose synthesis | Rnf, Mrp, Sud, HppA | NiFe hydrogenase (Group 4g), Mrp-linked |
| Orphnella | occulta T | 3300025586_21 (85%), 3300035698_104 (61%) | Sugars, 2-oxoacids | Trehalose synthesis | Rnf, Sud, HppA | NiFe hydrogenase (Group 3b)/sulfhydrogenase |
HppA, pyrophosphate-energized sodium pump; Mrp, multicomponent Na+:H+ antiporter; Nqr, sodium-translocating NADH:quinone oxidoreductase; Rnf, ferredoxin:NAD+-oxidoreductase complex; Sud, bifunctional sulfide dehydrogenase/ferredoxin:NADP oxidoreductase. TIndicates type species.