Table 2.
Summary of semen quality parameters during study period.
Semen Quality Parameters | Before (N*=74, n=219) |
During (N*=43, n=81) |
After (N*=726, n=1691) |
|
---|---|---|---|---|
Mean (SD) | Mean (SD) | Mean (SD) | p value | |
Abstinence (days) | 4.26 (1.19) | 4.48 (1.13) | 4.48 1.13) | 0.03 |
Semen Volume (mL) | 3.95 (1.66) | 3.98 (1.69) | 3.86 (1.43) | 0.55 |
Sperm Concentration (mil/mL) | 127.44 (65.24) | 136.91 (77.33) | 116.81 (73.58) | 0.01 |
Total Sperm Count (millions) | 476.48(262.48) | 514.85(307.98) | 430.49(271.45) | <0.001 |
Progressive Rate (%) | 70.31 (10.18) | 70.35 (8.57) | 66.52 (12.45) | <0.001 |
Total Motility(%) | 0.73(0.10) | 0.73(0.10) | 0.70(0.13) | <0.001 |
Round Cells(mil/mL) | 0.23 (0.20) | 0.22 (0.18) | 0.23 (0.52) | 0.97 |
VCL (μm/s)a | 46.22 (8.62) | 46.52 (9.00) | 44.50 (10.05) | 0.01 |
VAP (μm/s)a | 32.91 (6.34) | 33.32 (6.74) | 31.72 (7.36) | 0.02 |
VSL (μm/s)a | 24.47 (5.53) | 25.00 (5.93) | 23.83 (6.19) | 0.10 |
WOB (%)a | 0.63 (0.07) | 0.623 0.073) | 0.61 (0.09) | <0.001 |
STR (%)a | 0.60 (0.07) | 0.61 (0.07) | 0.59 (0.09) | <0.001 |
LIN (%)a | 0.44 (0.07) | 0.44 (0.07) | 0.43 (0.08) | 0.01 |
ALH (μm/s)a | 4.82 (1.04) | 4.62 (0.90) | 4.44 (1.08) | <0.001 |
BCF (times/s)a | 10.93 (1.71) | 11.48 (1.83) | 10.86 (2.04) | 0.02 |
Abbreviations: N=number of unique individuals; n=number of samples; SD=standard deviation; VCL=curvilinear velocity; VSL=straight-line velocity, VAP=average path velocity; ALH=amplitude of lateral head displacement; LIN=linearity; STR=straightness; WOB=wobble; BCF=beat-cross frequency.
* N is number of unique individuals during each time period, some individuals provided samples during different time periods; n is number of samples. Before is 2019–11-23 to 2020-01-23, During is 2020–01-24 to 2020-04-08, After is 2020–04-09 to 2020-07-23. **Test of significance at 0.05 level. N=unique individuals, n=number of samples.
Quality of sperm motion analyzed by CASA, only for samples with at least 25 sperm tracks, including VCL(μm/s), VAP(μm/s), VSL(μm/s), WOB(%), STR(%), LIN(%), ALH(μm/s), BCF(times/s).