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Ty1 insertional mutagenesis of CAN1
| Strain | Relevant genotype | Mutation ratea (108) | Ty1 fractionb | Estimated transposition ratec (108) |
|---|---|---|---|---|
| JC297 | RAD3 | 4.9 | 2/32 | 0.3 |
| BLY154 | rad3-G595R | 27.6 | 26/32 | 22.4 |
Rate of canavanine resistance per cell per generation was determined by the method of Drake (23).
Thirty-two independent can1 mutants were examined by PCR to determine whether a 2.3-kb region spanning the CAN1 gene contained a Ty1 insertion.
Product of mutation rate and Ty1 fraction.
