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. 2021 Oct 11;12:766170. doi: 10.3389/fimmu.2021.766170

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Copyright © 2021 Yamazaki, Kato, Tsuboi, Miyauchi, Suda, Sato, Nakajima, Yokoji-Takeuchi, Yamada-Hara, Tsuzuno, Matsugishi, Takahashi, Tabeta, Miura, Okuda, Kikuchi, Ohno and Yamazaki

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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P. gingivalis administration induces gut barrier dysfunction. (A) Expression of Tjp1 in the small intestines. cDNA was amplified with primers specific for Tjp1 (n=6-10/group). The relative quantity of mRNA was normalized to that of glyceraldehyde-3-phosphate dehydrogenase mRNA. (B) Immunofluorescence analysis of E-cadherin in large intestines from each group. Red: E-cadherin, blue: DAPI, scale bars: 50 μm. (C) Serum endotoxin levels in the various groups (n=6-10/group). Data are expressed as the mean ± standard error of the mean (SEM). P values were calculated using one-way ANOVA with Tukey’s multiple comparisons test. ***P < 0.001, ****P < 0.0001.