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. 2021 Oct 11;12:751883. doi: 10.3389/fimmu.2021.751883

Figure 1.

Figure 1

Ovalbumin fusion with FLIPr increases the deposition of ovalbumin on the nasal epithelium via intranasal administration. (A) Schematic position of NALT. Nasal cavity staining with hematoxylin and eosin. The red dotted line indicates the range of NALT, and the blue line indicates the epithelial layer that covers NALT. (B) PBS (left panel), rOVA (middle panel) or rOVA-FLIPr (right panel) was intranasally administered, and the nasal cavity was collected at the indicated time points. Antigen staining (green) in NALT was performed using an anti-His tag antibody following the Alexa Fluor®488-conjugated secondary antibody. Images were obtained from single optical sections acquired by means of confocal microscopy (40× total magnification, Scale bar = 100 µm). (C) The enlarged field of the white box from (B) (40× total magnification, Scale bar = 40 µm). Experiments were repeated twice with similar results.