Figure 2.

Ovalbumin fusion with FLIPr efficiently delivers to NALT dendritic cells via intranasal administration. (A) Gating strategy for the dendritic cell population in NALT. A group of C57BL/6 mice was intranasally administered 30 μg Alexa Fluor 647-labeled OVA or OVA-FLIPr. Mice administered PBS were used as control. Cells were harvested 2 hours after administration. A single-cell suspension of nasal cells (pooled from 3 mice) was obtained by mechanical disruption and collagenase digestion of nasal tissue. Dead cells were excluded from analysis by staining with a Live/Dead^® fixable dead cell stain dye. DCs from the NALT gated on live/CD45⁺/CD11c⁺MHCII⁺ cells. (B) The frequencies of antigen-labeled CD11c⁺MHC II⁺ cells. Cumulative data from three individual experiments are quantified here. The data are presented as the mean ± SEM (n = 3). Statistical significance was determined using the Kruskal-Wallis test with Dunn’s multiple comparison test. **p < 0.01; *** p < 0.001.