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. 2021 Oct 11;11:738706. doi: 10.3389/fcimb.2021.738706

Figure 4.

Figure 4

Hyper-biofilm-forming clinical strains have point mutation in the LeuO gene locus. (A) A. baumannii ATCC 17978 WT, ΔLeuO, and clinical strains of different sequence type ST-208(011), ST-229(079), ST-357(004), ST-451(001), ST-552(015), and ST-784(001) were cultured in 5-ml polystyrene tubes at 30°C for 24 h in LB broth without salt. (A) Biofilm formation on polystyrene tubes was photographed after staining with 0.1% crystal violet. (B) Cartoon representations of predicted LeuO gene structures of the clinical strains ST-208, ST-229, ST-357, ST-451, ST-552, and ST-784, respectively, obtained using the PyMoL software. Blue color indicates the N-terminal HTH domain, and green color indicates the C-terminal regulatory domain. Mutated residues are shown in red sticks at different positions such as 63, 99, 109, 187, 194, 195, 198, 264, and 303 and are marked beside.