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. 2021 Oct 11;12:733902. doi: 10.3389/fphar.2021.733902

FIGURE 2.

FIGURE 2

LincRNA00892 enhanced CD40L expression in Jurkat and primary CD4+ T cells. (A), (B): The expression of lincRNA00892 (A) and CD40L (B) in Jurkat and primary CD4+ T cells transfected with lentiviruses with control vector or vector containing the lincRNA00892 fragment was defined by qRT-PCR. (C) The protein levels of CD40L in the negative control and lincRNA00892 overexpression group in both Jurkat and primary CD4+ T cells were confirmed by western blotting. (D) The level of CD40L expressed on the surface of both Jurkat and primary CD4+ T cells in both negative control and lincRNA00892 overexpression group were performed by flow cytometry. NC = negative control, OE = overexpression, NS = no statistical difference. **p < 0.01, *p < 0.05. The results are expressed as mean ± standard deviation. Student’s t-test was used for comparison of two groups.