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. Author manuscript; available in PMC: 2022 Mar 21.
Published in final edited form as: Nat Microbiol. 2021 Sep 21;6(10):1233–1244. doi: 10.1038/s41564-021-00972-2

Fig. 3. Characterization of important sequence features of the AZD8895 public clonotype.

Fig. 3.

a. IMGT/DomainGapAlign results of AZD8895 heavy and light chains with germline V (IGHV1–58 and IGLV3–20), D (IGHD2–2, IGHD 2–8, or IGHD 2–15) or J (IGHJ3*02 and IGKJ1*01) gene segments and with representative variable gene sequences of mAbs in this public clonotype. Key interacting residues and their corresponding residues in germline genes are highlighted in yellow and colored in blue except for P99 in purple (heavy chain) or in red (light chain).

b. Binding curves of point mutants of AZD8895. Mutants of D108 residue are in blue, revertant mutation of inferred somatic mutations to germline sequence (GRev) are in green, P99 mutants are in orange, and C101A/C106A mutations removing the disulfide bond in HCDR3 is in purple. Data points show the mean ± SD for each tested antibody dilution. Experiments were performed in technical triplicate, with data shown from a single experiment repeated twice. Data for the AZD8895 wild-type binding curve shown in both panels are from the same experiment.