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. Author manuscript; available in PMC: 2021 Oct 25.
Published in final edited form as: Brain Behav Immun. 2018 Jul 29;73:670–681. doi: 10.1016/j.bbi.2018.07.021

Fig. 3.

Fig. 3.

HX600 protects neurons from LPS induced death. Primary microglia or BV2 cells were added on top of primary cortical neurons, and the co-cultures were pre-exposed to HX600 or vehicle for 6 h. After that the cultures received either vehicle or LPS/IFNγ treatment, while HX600 concentration was maintained at 1 μM. HX600 significantly prevented neuronal death. Representative images of the neurons stained with MAP-2 (A) and quantitative analysis (B) from a primary microglia – neuron co-culture. Transfection of BV2 cells with Nurr1 siRNA resulted in 40% knock-down of Nurr1 (C). When the neurons were co-cultured with Nurr1 siRNA transfected BV2 cells, the protective effect of HX600 against the inflammation induced neuronal death was abolished (D). Mock transfection of BV2 cells with control siRNA did not prevent the protection (E). Data presented as mean ± SD, 1-way ANOVA followed by Bonferroni posthoc test (B, D and E) and unpaired two-tailed T-test (C), n = 3–12 wells in each group, each assay repeated three times. *p < 0.05 **p < 0.01 ***p < 0.001.