Table 1.
Cytokines detected in CM from hMSCs exposed to ARDS and healthy volunteer BALF samples
| Cytokine | Cytokines in conditioned media (pg/ml) | Kruskal-Wallis with Dunn’s Compared to UNS | |||
|---|---|---|---|---|---|
| UNS | HC | ARDS | HC | ARDS | |
| Mean (SD) | Mean (SD) | Mean (SD) | P-value | P-value | |
| ADAMTS13 | 1.0 (0.0) | 1.0 (0.0) | 1.0 (0.0) | NA | NA |
| CXCL8/IL-8 | 20.9 (15.9) | 1227.8 (2307.8) | 2583.7 (4101.4) | 0.0084 (**) | 0.0008 (***) |
| Fas Ligand | 1.0 (0.0) | 1.3 (1.0) | 1.0 (0.0) | 0.3019 | >0.9999 |
| GM-CSF | 1.8 (1.0) | 2.5 (2.4) | 5.7 (9.3) | >0.9999 | 0.3280 |
| IL-10 | 1.0 (0.0) | 1.2 (0.6) | 1.0 (0.0) | 0.6403 | >0.9999 |
| IL-13 | 1.0 (0.0) | 1.0 (0.0) | 1.0 (0.0) | NA | NA |
| IL-2 | 1.0 (0.0) | 10.7 (22.5) | 23.7 (39.7) | 0.6189 | 0.2913 |
| IL-4 | 1.0 (0.0) | 6.2 (7.3) | 10.8 (10.6) | 0.2390 | 0.0457 (*) |
| Leptin | 1.0 (0.0) | 1.0 (0.0) | 1.0 (0.0) | NA | NA |
| MIF | 2056.0 (2742.6) | 20575.8 (30710.7) | 14619.7 (34703.7) | 0.2957 | 0.3239 |
| CCL4 | 1.0 (0.0) | 1.0 (0.0) | 96.6 (166.5) | >0.9999 | 0.1171 |
| Osteopontin | 7638.4 (937.2) | 9799.8 (3483.3) | 9289.5 (3196.5) | 0.4702 | 0.6699 |
| TNF-α | 1.0 (0.0) | 1.0 (0.0) | 1.0 (0.0) | NA | NA |
| CD44 | 121.7 (46.4) | 1508.0 (3486.9) | 1786.9 (3101.3) | 0.0342 (*) | 0.0041 (**) |
| Fas | 1.0 (0.0) | 47.3 (79.6) | 260.2 (493.0) | 0.1293 | 0.0477 (*) |
| G-CSF | 1.0 (0.0) | 1.0 (0.0) | 10.1 (24.0) | >0.9999 | 0.3809 |
| HGF | 15.8 (23.4) | 41.7 (45.7) | 68.0 (47.0) | 0.2356 | 0.0180 (*) |
| IL-1β | 1.0 (0.0) | 4.1 (9.8) | 32.0 (74.2) | >0.9999 | 0.2291 |
| IL-12 p70 | 11.9 (10.9) | 16.8 (29.4) | 8.7 (13.2) | >0.9999 | >0.9999 |
| IL-18 | 3.5 (4.4) | 49.9 (42.0) | 44.4 (45.5) | 0.0157 (*) | 0.0591 |
| IL-36β | 1.0 (0.0) | 1.3 (0.7) | 2.3 (2.3) | >0.9999 | 0.3667 |
| IL-6 | 140.0 (108.4) | 2202.3 (4080.5) | 4179.4 (7296.9) | 0.0257 (*) | 0.0034 (**) |
| CCL2 | 1268.4 (716.0) | 6290.1 (6588.2) | 4377.0 (5283.3) | 0.0208 (*) | 0.0903 |
| CCL3 | 1.0 (0.0) | 38.8 (67.3) | 47.6 (80.6) | 0.3145 | 0.3667 |
| MMP-3 | 1.0 (0.0) | 58.8 (117.8) | 372.4 (681.0) | 0.4444 | 0.0041 (**) |
| SP-D | 1.0 (0.0) | 163.8 (176.5) | 682.3 (13344.2) | 0.0055 (**) | 0.0162 (*) |
| IFN-γ # | 0.07 (0.14) | 1.5 (2.2) | 0 (0) | 0.9276 | >0.9999 |
Cytokines detected in conditioned media from hMSCs exposed to ARDS BALF samples (n=7), healthy control BALF samples (n=10), or PBS-exposed (n=7) using 27-plex Luminex assay. Samples were analyzed on two luminex plates. Mean with SD of extrapolated values are presented. Values out of range below were set to 1.0. IFN-γ was measured on a separate ELISA on conditioned media from hMSCs exposed to ARDS BALF samples (n=3), healthy control BALF samples (n=5), or PBS-exposed (n=4). Data are presented as mean with SD. Kruskal-Wallis tests (Dunn’s post hoc test) was used to assess differences between ARDS/HC exposed hMSCs and unstimulated hMSCs. CM, conditioned media; hMSCs, human mesenchymal stromal cells; BALF, bronchoalveolarlavage fluid; UNS, unstimulated; ARDS, acute respiratory distress syndrome; HC, healthy control subjects;
IFN-γ was measured on a separate ELISA; SD, standard deviation; NA, not available;
≤ 0.05;
≤ 0.01,
≤ 0.001.