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. Author manuscript; available in PMC: 2021 Oct 25.
Published in final edited form as: Cell Chem Biol. 2018 Jan 27;25(4):357–369.e6. doi: 10.1016/j.chembiol.2017.12.014

Figure 1. Identification of Dynarrestin.

Figure 1.

(A) Schematic of the assay for embryoid body (EB)-based differentiation of mouse embryonic stem cells (mESCs) into motor neurons in an Hh-dependent manner.

(B) Time course of Hb9:GFP expression under the indicated conditions.

(C) Chemical structure of dynarrestin.

(D) Effect of the indicated compound on Hb9:GFP intensity in treated EBs (Day 5).

(E–G) Chemical structure of dynarrestin analogs (E). Effects of the indicated compounds in assays of (F) Hh-dependent osteogenesis of C3H10T1/2 cells and (G) luciferase activity using Shh-LIGHT2 cells.

Error bars indicate data range. See also Figures S1 and S2.