KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dynarrestin | This paper | N/A |
| Vismodegib | Selleckchem | Cat#S1082 |
| Purmorphamine | Cayman Chemical | Cat#10009634 |
| CDP-Star | Roche | Cat#12041677001 |
| Smoothened agonist | Cayman Chemical | Cat#11914 |
| Rhodamine-labeled tubulin | Cytoskeleton | Cat#TL590M |
| Human α2-macroglobulin | Calbiochem | Cat#441251 |
| Ciliobrevin A | Sigma | Cat#H4541 |
| Ciliobrevin D | Calbiochem | Cat#250401 |
| Tetramethylrhodamine, ethyl ester (TMRE) | ThermoFisher Scientific | Cat#T669 |
| Experimental Models: Cell Lines | ||
| Hb9::GFP mouse ESCs | (Höing et al., 2012) | N/A |
| Shh-light 2 cells | ATCC | ATCC JHU-68 |
| C3H/10T1/2 | ATCC | ATCC CCL-226 |
| Cos-7 cells | ATCC | ATCC CRL-1651 |
| IMCD3 cells | ATCC | ATCC CRL-2123 |
| NIH-3T3 cells | DSMZ | DSMZ ACC 59 |
| Antibodies | ||
| Rabbit anti-Smoothened | Abcam | Cat#ab38686; RRID: AB_882615 |
| Mouse anti-acTubulin | Sigma | Cat#T6793; RRID: AB_477585 |
| Goat anti-Rabbit Alexa594 | Invitrogen | Cat#A11012; RRID: AB_10562717 |
| Donkey anti-Mouse Alexa488 | Invitrogen | Cat#A21202; RRID: AB_141607 |
| Rat anti-Tubulin | Serotec | Cat#YL1/2; RRID: AB_325003 |
| Mouse anti-Dynein | Millipore | Cat#DIC74.1; RRID: AB_2246059 |
| Mouse anti-Tubulin | Sigma | Cat#DM1A; RRID: AB_477593 |
| Recombinant DNA | ||
| pCAG-IFT88–GFP | (He et al., 2014) | N/A |
| pCAG-ARL13b–mCherry | (He et al., 2014) | N/A |
| Critical Commercial Assays | ||
| One Glo | Promega | Cat#E6110 |
| Cell Titer Glo | Promega | Cat#G7570 |