Fig. 2.

Overexpression of circ_0004296 inhibited the proliferation, migration, invasion, and EMT of PCa cells. A Schematic illustration of the circ_0004296 plasmid (pLenO-GTP- circ_0004296) or empty vector. B The expression of circ_0004296 and ETS1 was detected by qRT-PCR in PC3 and DU145 cells transfected with the circ_0004296 plasmid or empty vector. C, D CCK8 and colony formation assays were used to measure viability of PCa cells after circ_0004296 overexpression. E-H The migration and invasion abilities of PCa cells were analyzed by wound healing and Transwell assays for migration and invasion after circ_0004296 overexpression. Scale bars =100um. I Western blotting assay was performed to detect the expression of EMT-related proteins, namely E-cadherin, N-cadherin, Vimentin, and Snail, after circ_0004296 overexpression. J IF assays showed that circ_0004296 overexpression increased epithelial marker (E-cadherin) expression but reduced mesenchymal marker (Vimentin) expression in PCa cells. Scale bars =20um. *P < 0.05, **P < 0.01, ***P < 0.001