Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 25;40:336. doi: 10.1186/s13046-021-02138-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — EIF4A3 protein mediated circ_0004296-induced inhibition of host gene ETS1 expression in PCa cells. A, B Intracellular distribution of circ_0004296 was detected by RNA-FISH and nuclear-plasma extraction assay. Scale bars =50um. C The circ_0004296 overexpression decreased the expression of the ETS1 protein. D circ_0004296 knockdown increased the expression of the ETS1 protein. E Schematic illustration of RNA pull-down for circ_0004296. F Schematic exhibiting the overlap for specific proteins bound by circ_0004296 via protein profiling and the potential target genes of circ_0004296 predicted by CircInteractome and CircFunBase. G RNA pull-down and western blotting assays confirmed the interaction of circ_0004296 with EIF4A3. H The efficiency of EIF4A3 knockdown at mRNA level. I The efficiency of EIF4A3 knockdown at protein level. J EIF4A3 knockdown decreased ETS1 expression. *P < 0.05, **P < 0.01, ***P < 0.001