Fig. 4.

EIF4A3 knockdown promoted the proliferation, migration, invasion, and EMT induction of PCa cells. A GSEA of TOMLINS_METASTASIS_UP and SUNG_METASTASIS_STROMA_UP GSEA gene sets referred to as metastasis-related gene signatures; NES, normalized enrichment score. B EIF4A3 expression was detected by qRT-PCR in PCa cell lines, PCa tissues, and adjacent normal tissues. C, D CCK8 and colony formation assays were used to measure viability of PCa cells after EIF4A3 knockdown. E-H The migration and invasion abilities of PCa cells were analyzed by wound healing and Transwell assays for migration and invasion after EIF4A3 knockdown. Scale bars =100um. I Western blotting assay was performed to detect the expression of EMT-related proteins, namely E-cadherin, N-cadherin, Vimentin, and Snail, after EIF4A3 knockdown. J Kaplan-Meier analysis for different EIF4A3 protein expression in PCa patients. K EIF4A3 expression in PCa tissues and normal samples from the TCGA database. L EIF4A3 expression was associated with disease-free survival of 492 patients with PCa from the TCGA database. *P < 0.05, **P < 0.01, ***P < 0.001