Fig. 5.

Circ_0004296 inhibited nuclear export of ETS1 mRNA through interaction with EIF4A3. A GSEA of GO_NUCLEAR_EXPORT and GO_RNA_SPLICING functional signatures in TCGA databases; NES, normalized enrichment score. B-D Knockdown or overexpression of circ_0004296 had no effect on the expression of EIF4A3 in PCa cells. E, F The levels of nuclear and cytoplasmic EIF4A3 protein were determined by western blotting assay after circ_0004296 overexpression. G, H The levels of nuclear and cytoplasmic ETS1 mRNA were determined by qRT-PCR after circ_0004296 overexpression. I, J RIP assay showed that EIF4A3 interacted with circ_0004296 and ETS1 mRNA in PCa cells. *P < 0.05, **P < 0.01, ***P < 0.001