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. 2021 Oct 25;40:336. doi: 10.1186/s13046-021-02138-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Circ_0004296 inhibited PCa growth and metastasis in vivo. A, B The subcutaneous xenograft mouse model showed that overexpression of circ_0004296 inhibited PCa growth in vivo (DU145-Vector, n = 6; DU145-circ_0004296, n = 6). C H&E staining and IHC assay showed that circ_0004296 overexpression downregulated the Ki67 protein level (magnification 200×). D The orthotopic xenograft mouse model showed that overexpression of circ_0004296 suppressed PCa growth and pelvic peritoneal invasion. E IHC assay showed that circ_0004296 overexpression remarkably downregulated mesenchymal marker (Vimentin) protein level but upregulated epithelial marker (E-cadherin) protein level (magnification 200×). F IHC assay showed that overexpression of circ_0004296 remarkably reduced the ETS1 protein level (magnification 200×). G IF assay showed that the expression of EIF4A3 protein from the circ_0004296 overexpression group was increased in the nucleus as compared to that in the control group. (magnification 200×). H The in vivo tail vein injection model showed that overexpression of circ_0004296 suppressed metastasis of PCa. I Kaplan–Meier analysis showing survival of mice with intravenous xenograft (DU145-Vector, n = 8; DU145-circ_0004296, n = 5). P value was determined using a log-rank test. *P < 0.05, **P < 0.01, ***P < 0.001