Fig. 2.

CircRNA NRIP1 directly sponges miR-195-5p and negatively regulated its expression, and miR-195-5p levels were reduced in stage III/IV PTC tissues and CircRNA NRIP1 was negatively correlated with miR-195-5p expression in PTC patients. A The predicted interaction sites of miR-195-5p and CircRNA NRIP1. B Analysis of the influence of CircRNA NRIP1  on miR-195-5p  expression using dual-luciferase reporter assay in TPC1 and IHH-4 cells. C Pull-down assay for analyzing the interaction relationship between labeled miR-195-5p and CircRNA NRIP1 in TPC1 and IHH-4 cells. D The expression levels of miR-195-5p in TPC1 and IHH-4 cells following the transfection of with CircRNA NRIP1, sh-NRIP1, miR-195-5p or its controls were detected through qRT-PCR. E The expression levels of miR-195-5p in stage I/II PTC patients and stage III/IV PTC patients via qRT-PCR. F Pearson’s correlation coefficient was applied to analyze the relationship between CircRNA NRIP1 and miR-195-5p expression in PTC patients. *P < 0.05