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. 2021 Oct 25;40:335. doi: 10.1186/s13046-021-02135-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — PCI-24781 does not inhibit HDAC11. A GEPIA on HDAC expression in GBM (Sample size; Control (Black) (n = 207) and GBM (Red) (n = 163)). HDAC1 and 2 are significantly upregulated, while HDAC11 is downregulated (p < 0.01). GEPIA- Gene Expression Profiling Interactive Analysis. B–H HDAC1, HDAC2, and HDAC11 assay were done by Reaction Biology Corp. using fluorogenic peptide from p53 residues 379–382 (RHKK(Ac)AMC) [substrates for HDAC1 and 2] and trifluoroacetyl lysine [substrate for HDAC11]. PCI-24781 and vorinostat inhibit HDAC1 and 2 but do not have an inhibitory effect on HDAC11, as do belinostat and TSA. B, D & F HDAC reference compound TSA were tested in a 10 dose IC₅₀ with 3 fold serial dilution starting at 10 μM (C) HDAC1, (E) HDAC2 and (G) HDAC11. (H) IC₅₀ values are summarized in table.*Empty cells indicate no inhibition or compound activity that could not fit an IC₅₀ curve. TSA-Trichostatin