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. 2021 Oct 25;40:335. doi: 10.1186/s13046-021-02135-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — PCI-24781 induced DNA DSB (γH2AX foci) in GBM cells. A GBM cells were incubated with vehicle control, TMZ, PCI-24781, and combination for 48 h and probed with γH2AX (phospho S139) antibody, and nuclei counterstained with DAPI. Representative immunofluorescence images are shown. Scale Bar 5 μm. B Quantitative measurement of γH2AX staining represented as an arithmetic mean intensity per cell. Staining data were compared between control and treatment groups using one-way ANOVA. Pairwise comparisons between control and treatment groups were adjusted for multiple comparisons with Tukey’s method. “*” significantly different compared to vehicle control; ‘*’ p < 0.05; ‘$’ p < 0.001; ‘#’ p < 0.0001. “$” significantly different compared to TMZ; “#” significantly different compared to PCI-24781. C Immunoblot analysis of γH2AX (phospho S139) levels in vehicle or drug-treated GBM cells. DNA DSB- DNA double-strand break