TABLE 2.
Limit of detection of E. histolytica-E. dispar by BIOSITE Triage IC strip and Alexon-Trend ProSpecT ELISA
| No. of HM1:IMSS trophozoites/ml | OD in Alexon-Trend ProSpecTa | BIOSITE Triage IC strip resultb
|
|
|---|---|---|---|
| Before filtration | After filtration | ||
| 105 | 1.481 (+) | +++ | ++ |
| 104 | 0.883 (+) | ++ | + |
| 103 | 0.264 (+) | + | +/− |
| 500 | 0.253 (+) | +/− | − |
| 250 | 0.169 (+) | − | − |
| 100 | 0.088 (−) | − | − |
| 10 | 0.066 (−) | − | − |
a
An optical density (OD) of >0.100 was regarded as positive (+) for ProSpecT in this assay run.
b
Filtration of stool specimens resuspended in specimen dilution buffer (protein-buffered solution containing 0.1% NaN3) was carried out with the filter devices provided in the Triage kit according to the manufacturer’s recommendations (Biosite Diagnostics). Band intensity with the Triage kit was measured visually by two readers and scored as follows: +++, very strong; ++, strong; +, medium; +/− weak; − none.