Inhibition of mitochondrial CaMKII suppressed autophagy. A, Adenovirus containing the cDNA for mitochondrial-targeted GFP (empty vector) or CaMKIIN. B, HBECs were transfected with or without Mt-CaMKIIN and confirmed by immunostaining. C, Representative immunofluorescence images of LC3B, ATG5, and p62 expression (red) in empty-GFP or Mt-CaMKIIN–transfected HBECs with PBS or cockroach allergen treatment. D, Quantitative analysis of florescent signals (n = 6). Data represent mean ± SEM. *P < .05, **P < .01, ***P < .001.