Inhibition of mitochondrial CaMKII-suppressed autophagy. A, Western blot analysis of LC3-I, LC-3II, ATG5, and p62 in empty-GFP or Mt-CaMKIIN–transfected HBECs with PBS or CRE treatment. β-Actin was measured as the loading control. B, Quantitative analysis of Western blots (n = 3). C, Western blot analysis of p-AMPK, AMPK, p-BECLIN 1, BECLIN 1, p-mTOR, and mTOR expression in empty-GFP or Mt-CaMKIIN–transfected HBECs with PBS or CRE treatment. β-Actin was measured as the loading control. D, Quantitative analysis of Western blots. p-AMPK, Phosphorylation of AMPK; p-MTOR, phosphorylation of mTOR. Data represent mean ± SEM of 2 independent experiments. *P < .05, **P < .01, ***P < .001.