TABLE E1.
Antibodies used for WB and immunostaining
| Target | Specie | Clone | Assay (dilution) | Company |
|---|---|---|---|---|
| AMPK | Rabbit | D5A2 | ● WB (1:500) | Cell Signaling |
| ATG5 | Rabbit | NB110-53818 | ● WB (1:500) ● IF (1:300) |
NOVUSBIO |
| BECLIN-1 | Rabbit | 3738 | ● WB (1:500) | Cell Signaling |
| Ep-CAM | Mouse | VU1D9 | ● IF (1:500) | Cell Signaling |
| GAPDH | Rabbit | 14C10 | ● WB (1:1000) | Cell Signaling |
| GFP | Rat | FM264G | ● IF (1:500) | BioLegend |
| LC3A/B | Rabbit | NB100-2220 | ● WB (1:1000) ● IF (1:200) |
NOVUSBIO |
| mTOR | Rabbit | 7C10 | ● WB (1:500) | Cell Signaling |
| ox-CAMKII | Rabbit | 07-1387 | ● WB (1:500) ● IF (1:100) |
EMD Millipore |
| p-AMPK | Rabbit | D4D6D | ● WB (1:500) | Cell Signaling |
| p-BECLIN-1 | Rabbit | D9A5G | ● WB (1:500) | Cell Signaling |
| p-mTOR | Rabbit | D9C2 | ● WB (1:500) | Cell Signaling |
| p62 | Rabbit | NBP1-48320 | ● WB (1:2000) | NOVUSBIO |
| PARKIN | Mouse | 4211 | ● WB (1:1000) | Cell Signaling |
| PINK1 | Rabbit | 6946 | ● WB (1:500) | Cell Signaling |
| TOM20 | Mouse | F-10 | ● IF (1:200) | Santa Cruz Biotechnology |
| β-Actiin | Mouse | 2F1-1 | ● WB (1:1000) | BioLegend |
GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; p-AMPK, phosphorylation of AMPK; p-MTOR, phosphorylation of mTOR; WB, Western blot.