FIG. 1.

Dox rescues induction to endoderm. (A) Schematic of C-MYC or BCL-XL Dox-inducible transduction and establishment of iPSC lines that were then able to efficiently form definitive endoderm. (B) Immunofluorescence and bright-field images showing induction of definitive endoderm from iPSCs after 6 days using the protocol shown in Supporting Fig. S1A, with and without Dox in iPSCs with established Dox-inducible C-MYC, BCL-XL, or control (no viral transduction) in cell line AS7017-5 (scale bar = 100 μM). Control experiments without a virus rescued the induction of cell line AS7017-5 (n = 2). (C) Bright-field images from three inefficient iPSC lines induced with or without the addition of Dox (no additional transgenes); scale bar = 100 μM (n = 3). (D) Schematic for optimal induction of iPSCs into definitive endoderm (days 0–7) and then to iPSC-Heps. Protocol also referred to as ML1. Abbreviations: HGF, hepatocyte growth factor; iHep, induced hepatocytes; OsM, oncostatin M.