Fig. 1.

Visualization of the accumulation of AAGG repeats and heterochromatin in selected individuals of P. vitticeps (PVI; A-L), P. henrylawsoni (PHE; M-P), and P. minor (PMI; Q-T). In P. vitticeps, the figure represents successively a standard male ZZ karyotype with no accumulations (A, B), standard female ZW karyotype with AAGG (C) and heterochromatin accumulation (D), karyotype of a male with the W-specific molecular marker amplified by primers F4/F1 and with AAGG accumulation (E) but without heterochromatinization (F), karyotype of a sex-reversed ZZ female with no accumulations (G, H), karyotype of a male positive for the molecular marker and no accumulations (I, J), and karyotype of a female with 2 AAGG accumulations (K) and unpaired heterochromatic block (L). In P. henrylawsoni and P. minor, male karyotypes with no accumulations (M, N, Q, R) and female karyotypes with AAGG (O, S) and heterochromatin accumulations (P, T) are shown. Boxes in K show the W chromosome and the chromosome with the AAGG accumulation inherited from the father in separated blue channel mode to present their size difference. Scale bars, 10 ìm.