Silver-stained SDS-PAGE gel of whole-cell lysates of B. burgdorferi sensu lato strains. Lanes 1 to 5, B. burgdorferi sensu stricto B31, B. garinii 20047, B. afzelii VS461, B. valaisiana AR-2, and Borrelia isolate A14S, respectively. The positions of flagellin (Fla), OspA, OspB, and OspC are indicated by arrows. The unique protein bands of isolate A14S are marked with asterisks. Molecular mass standards (Pharmacia) are shown on the right.