FIG 5.

Measurements of relative mRNA levels of catabolic genes by RT-qPCR. Strains SK36 (MMZ1896), ccpA, manL, and manL ccpA were each cultured, in a TY medium containing 20 mM glucose, to the exponential phase before being harvested for RNA extraction. An internal control (gyrA) was used to measure the relative abundance of each transcript. The results for each gene are presented as the average and standard deviation (error bar) of three biological repeats. Asterisks represent statistical significance relative to the wild type, assessed by a one-way ANOVA test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).