Table 1.

Description of novel mouse strains expressing human LOAD risk alleles.

Common name	JAX stock #	Strain	Background	Gene location	Allele name	Allele type	Additional considerations
B6	000664	C57BL/6J	-	-	-	-	-
Trem2*R47H	027918	C57BL/6J- Trem2em1 Adiuj/J	C57BL/6J	Chr17:4834 6401- 48352276	Trem2 R47H KI	Cas9 endonuclease- mediated (humanized sequence)	- Two silent mutations (lysine AAG>AAA and alanine GCC>GCA) into Trem2 - R47H mutation also introduces a cryptic splice acceptor site in exon 2, creating a novel splice variant with a deletion of 119bp at the 5’ end of exon 2.
APOE4	027894	B6(SJL)- Apoetm1.1(APOE*4) Adiuj/J	C57BL/6J	Chr7:19696 109-19699188	APOE4 KI	FRT site flanked PGK- neo cassette targeted mutation (gene replacement)	- Exons 2, 3, and a majority of exon 4 of the mouse Apoe gene were replaced by exons 2, 3, and 4 of the human APOE gene sequence (including a portion of the 3’ UTR sequence) - Expression of FLP recombinase was used to remove the FRT site flanked PGK-neo cassette and subsequently backcrossed to remove FLP recombinase
APOE4. Trem2*R47H	028709	B6(SJL)- Apoetm1.1(APOE*4)Adiuj Trem2em1Adiuj/J	C57BL/6J	(see above)	(see above)	(see above)	(see above)

Mouse strains expressing Trem2*R47H and APOEε4 human late-onset Alzheimer Disease risk factors, alone or in combination, developed on the C57BL/6J background and distributed by The Jackson Laboratory (https://www.jax.org/mouse-search).