From the microbial perspective, accessing a terminal electron acceptor to
power metabolism is a problem that transcends context. The flavin EET pathway
has been elucidated as a specific solution that provides an advantage in the
anaerobic intestinal tract for L. monocytogenes, whose natural
ecological range is broad, encompassing soils to host-associated environments.
These environments often contain complex microbial communities and diverse
molecules that may influence EET metabolism. Light et al. (Light et al.,2018) propose that EET proceeds through
an alternative membrane-bound electron transport chain to the outside of the
cell. This electron transport chain consists of an NADH dehydrogenase (Ndh2),
specific menaquinone derivatives (DMK), possibly two membrane proteins (EetA and
EetB), and an outward facing lipoprotein (PplA) with covalently attached flavins
that serve as electron carriers. Their experiments provide evidence that flavins
are sufficient to serve as extracellular electron shuttles from PplA to
iron(III) and electrodes, but many questions remain, e.g.: (1) what is the
terminal electron acceptor in any given niche? (2) What EES are involved in long
range electron transfer? (3) How does EET contribute to energy generation within
the cell?