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. 2021 Oct 13;15(10):e0009862. doi: 10.1371/journal.pntd.0009862

Fig 2. Laboratory analysis.

Fig 2

Microscopic examination of swab smears (A1 –A3) and histological sections (B1 –B4) of biopsies from wounds suspected to be BU lesions. Specimens were either stained with ZN-Methylene blue (A1-A3 and B2-B4) to detect AFBs (pink) and nucleic acid, implicating DNA from secondary infections (blue); or with Haematoxylin-Eosin (B1). A1-A3: Staining of wound exudate from different swabs revealed a heavy infection with rods and/or cocci but no AFBs. A1: rods and cocci (participant 14). A2: rods (participant 11). A3: cocci and leukocytes (participant 4). B1: overview of excised tissue specimen revealing a secondary infection at the open ulcer surface and the underlying tissue (participant 21). B2: higher magnification of this area revealed the presence of rods but no AFBs (participant 21), B3: the same higher magnification at a different area revealed a strong infection with cocci but no AFBs (participant 21) B4: staining of tissue sections from a different participant revealed the presence of large numbers of rods but again no AFBs (participant 6).