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. 2021 Oct 25;16(10):e0258711. doi: 10.1371/journal.pone.0258711

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© 2021 Van Reet et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — All Trypanozoon strains were tested at 50 ng of pure genomic DNA. Mean and standard deviation were calculated using three replicates for each sample. RCNs for 18S rDNA were calculated using the ΔC_q-method between q18S and qGPI-PLC. RCNs for the TBR targets q177_D, q177_T, qM and q176_T were calculated using the ΔC_q-method with q18S as reference.