FIG 8.

IL-12p40-mediated activation of autophagy depends on the JAK-STAT pathway. (A) Status of pJAK2 and pSTAT4 in infected macrophages after treatment with DP and CI994. (B and C) Densitometric analysis of pJAK2 (B) and STAT4 (C) bands normalized with that of β-actin. Each value represents mean ± SD from triplicate measurements. *, band intensity is significantly different from infected (IF) samples individually treated with inhibitors, respectively; P ≤ 0.05. (D) Infected THP-1-derived macrophage cells were treated with JAK2 inhibitor NSC33994 along with DP and CI994. THP-1-derived macrophages deficient in STAT4 were treated with DP and CI994. Intracellular bacterial viability was determined based on the number of CFU. Isolation of bacilli from the macrophages was carried out at 24 h. The number of viable bacilli in each of the plate was assayed by plating lysed macrophages on 7H10 agar plates and incubating the plates at 37°C for 3 weeks and counting the CFU. Each value represents mean ± SD from triplicate measurements. *, survival is significantly different from infected (IF) sample; P ≤ 0.05.