FIG 5.

Sensitization and challenge with W72310 cause increased pulmonary inflammation, leakage, and mucous cell metaplasia. (A) Total BAL fluid was quantified for individual cell populations of macrophages, neutrophils, lymphocytes, and eosinophils. (B) Paraffin-embedded fixed lungs were stained with H&E and PAS, and images were captured at 20×; scale bar = 100 μm. (C) BAL supernatant was analyzed for total concentrations of albumin. (A and C) Kruskal-Wallis with Dunn’s comparison to 2-day time point (within same sensitization protocol): *, ≤0.05. Kruskal-Wallis with Dunn’s comparison to same time point (within different sensitization protocol): #, ≤0.05. Data include 2 independent experiments and n = 8 to 12 mice.