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. 2021 Feb 10;6(1):e01318-20. doi: 10.1128/mSphere.01318-20

TABLE 2.

Cdr1 expression levels and ATPase activities of plasma membrane preparations isolated from ADΔΔ (negative control) and from ADΔΔ strains overexpressing CDR1P-GFP (positive control) and the CDR1PC-GFP Cys-deficient variants

Strain no. Straina ATPase (nmol/min/mg)b,c MICFLC (mg/liter) Cdr1 expressiond Normalizede
ATPase MICFLC
CDR1P 307 (14) 256 100 100 100
1 N1 440 (25) ***g 512 110 130 *** 180
2 TS1 92 (20) *** 64 110 30 *** 20
3 EL3 228 (42) * 64 100 80 * 30
4 T1 − 16 70 − 10
5 NTS1 200 (28) ** 64 110 60 *** 20
6 NT1 − 4 60 − 3
7 N2f 65 (19) *** 256 30 70 * 310
8 TS2 18 (9) *** 128 100 10 *** 50
9 EL6 − 1 70 − −
10 T2 − 1 40 − −
11 NTS2f 11 (8) *** 32 30 10 *** 40
12 NT2f − 1 20 − −
13 N12f 75 (10) *** 128 20 120 * 240
14 TS12 − 16 100 − 6
15 EL36 20 (3) *** 1 50 10 *** −
16 T12 − 1 40 − −
17 NTS12f − 8 20 − 10
18 NTS12-S1f 64 (11) *** 128 60 40 *** 90
a

The strain symbols are the same as in Table 1.

b

The ATPase activities were corrected for the oligomycin-sensitive ADΔΔ background (22 ± 10 nmol/min/mg); values are the means (±SDs) of two technical replicates measured three times.

c

The ATPase activities with a − sign were below the detection limit of the assay.

d

Cdr1 expression levels (% relative to wild-type Cdr1P-GFP).

e

Cdr1 ATPase activities and MICFLCs (% relative to wild-type Cdr1P-GFP) normalized with respect to the different expression levels.

f

Heat shock protein SSA2 was upregulated in these strains (Fig. 4 and text give further details).

g

Significant differences between the Cdr1 ATPase activities were determined with the Student t test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.