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Cartoon representation depicting a workflow of the transduction procedure developed for introduction of phagemids in Sodalis species. Following the direction of the arrows, an E. coli P1 lysogen host is transformed with a P1 phagemid. The phagemid is packaged following induction of the P1 prophage, and lysates derived from culture supernatant are used to infect a Sodalis recipient strain. Cells receiving the phagemid are subsequently isolated on plates containing a selective agent.
