FIG 9.
DSF thermal shift measurement of DBOs binding to PBP3. (A) Zidebactam, WCK 5153, and WCK 4234 binding to PBP3 at the indicated concentrations. The compounds were dissolved in DMSO with a final DMSO concentration of 2% in the DSF experiment; a PBP3 2% DMSO control experiment is therefore also included. For comparison, ceftazidime is a positive control since it showed a large increase in Tm as previously published (29). Experiments were done in duplicate, and a representative curve is shown for each experiment. (B) Avibactam binding to PBP3. Data are plotted similarly as in Fig. 5. Avibactam was dissolved in water. Experiments were done in triplicate, and a representative curve is shown for each experiment.