TABLE 1.
Phenotype summary
| Phenotype | Value or description for phosphosite: |
||||
|---|---|---|---|---|---|
| WT | S262A | S268A | T308A | S319A | |
| Phosphosite conservationa | F (all tested) | F (A. nidulans) | F and Y (C. neoformans, C. albicans) | F (A. nidulans, T. reesei, F. graminearum) | |
| Radial growth, allyl alcohol (40 mM) (%) | 44.68 | 8.96 | 10.02 | 10.64 | 54.76 |
| Radial growth, 2DG (0.25 mM) (%) | 33.67 | 38.1 | 32.6 | 49.57 | 34.04 |
| Nuclear CreA-GFP (glucose) (%) | 93.52 | 70.2 | 77.9 | 16.41 | 93.88 |
| ADH activity (glucose+ethanol) (mU × mg/mg protein) | 3.47 | 5.43 | 2.43 | 4.35 | 4.15 |
| RT-qPCR for xlnA (30-min glucose+xylose) (fold increase) | 140.11 | 443.35 | 646.46 | 886.86 | 246.05 |
| Xylanase activity (72-h glucose+xylose) (mU × mg/mg protein) | 11.82 | 21.43 | 22.38 | 35.9 | 14.56 |
| Stability of CreA protein (30-min glucose) (GFP/Coomassie ratio) | 0.16 | 0.08 | 0.07 | 0.81 | 0.88 |
| Glucose supernatant (%) (17-h transport) | 5.74 | 17.77 | 32.56 | 11.96 | 5.74 |
| Glycogen (mg/mg of protein) | 0.046 | 0.068 | 0.048 | 0.071 | 0.01 |
| Trehalose (mg/mg of protein) | 0.038 | 0.028 | 0.013 | 0.017 | 0.045 |
a
F, filamentous fungi; Y, yeast.