TABLE 2.
Primers used in this study
| Use and/or construct | Sequence (5′–3′)a |
|---|---|
| Mutant construction | |
| gsp deletion | TTCTGAATTCCCGTCGTAAATTCTAACT |
| ATTTTTGGTTTAAGCGTGTTTTATAAACCTCCTTAG | |
| CTAAGGAGGTTTATAAAACACGCTTAAACCAAAAAT | |
| TTCTGGGATCCTTTATTCAGCATAGTCGC | |
| blpH deletion | TTCTGAATTCGTTTTACTGATGCCACTG |
| AGATGAGGTGAAAAGCTACTGAAATAGTCGAATGAT | |
| ATCATTCGACTATTTCAGTAGCTTTTCACCTCATCT | |
| TTCTGGGATCCCAAGGAATTGATGTCGCT | |
| blpR deletion | TTCTGAATTCCTTCCATACCTGTTAGAA |
| GATTTTTAGAGGAGATTTGGTGAAAAGCTAATGAGC | |
| GCTCATTAGCTTTTCACCAAATCTCCTCTAAAAATC | |
| TTCTGGGATCCATCTTTTCTCTAATATGG | |
| blpS deletion | TTCTGAATTCCAGCAGGAGAGGTTTCAA |
| GGAGGGAATAATGATTTATGGACTGATTTTTAGAGGAG | |
| CTCCTCTAAAAATCAGTCCATAAATCATTATTCCCTCC | |
| TTCTGGATCCTTGGCGAAAAAATCCTCG | |
| Promoters | |
| gsp promoter | TTCTGAATTCTTTTCAACTCATAACGAA |
| TTCTGGATCCTTTTATAAACCTCCTTAG | |
| gllA promoter | TTCTGAATTCGGTCCCAATCTCCCTT |
| TTCTGGATCCTTGAATACCTCCCAAT | |
| qPCR | |
| rpoB | CACCGTACACGTCGTAGC |
| CCGTAAAGTTTGTAATCG | |
| gallo_rs03700 | CGAGGTATCCTTTTGTGT |
| GGTATCACTCATAATTCC | |
| gallo_rs10335 | TTCCAACCTATACGCATG |
| AGCTTGTTGAATGAAGGC | |
| gsp | GATGACAGAAAAAATGTT |
| GTGTTTAGTAGGCTTATG | |
| blpH | ATGTTAGAGGAGCAAAGC |
| ACTCTCTATAACCCATGG | |
| blpR | AGAGGTGTTTAGTTCCGC |
| CTACTAACGCTTGGTAGG | |
| blpS | GGCTATTGACGATATCCT |
| GTCGCTGCTCTCTATCCA | |
| gallo_10370 | CTGGCTCATCTGATGTGTC |
| GTGCCTACAACTGAAACGA | |
| gllA2 | GAAGGTGGTTACAGCAAGACAG |
| CTACACAAGTAGCCCCACCAC | |
| blpA | CTCGCTGGCTCATTTGAG |
| GCGGGAGTTTGCCTTCTT | |
| gallo_rs10400 | GGCGTTTTTGGTAGCATTA |
| CAGCAGATAGTAAGCAATC | |
| Overexpression | |
| blpS | TTCTCTGCAGCTCCTCTAAAAATCAGTC |
| TTCTGGATCCGTTATTGGAGGGAATAATG | |
| RACE-PCR | |
| gllA | TACACCCGCCAATAGCAG |
| CCACCACTAATTGTTTGC | |
| His-tagged BlpR and BlpS | |
| BlpR | TTCTGGATCCTCACCTCATCTCATTTAA |
| TTCTCATATGATGTTAGATATTTATGTA | |
| BlpS | TTCTGGATCCTCAGTCATTTGAGATGAT |
| TTCTGCTAGCATGAAATATTTTAAATTTAC | |
| EMSA promoters | |
| gsp | TTCTGGATCCTCATTTTTATAAACCTCC |
| TTCTGAATTCGATGGCTTGGACTTTTTC | |
| blpA | TTCTGGATCCCTTCTCATAACCTTTCCC |
| TTCTGAATTCTTTGGAAGAATGGTAAAG | |
| gyrA | TTCTGGATCCTAAGGAAAAACACTCCTT |
| TTCTGAATTCTAAGTGAGATATGTCACG | |
| gllA | TTCTGGATCCATAATATTTTGTCGTTGC |
| TTCTGAATTCACGGTCAAAAAATCATGA | |
| pTCV-lac primers | |
| VlacE | GAGTCAAAATAGATATGAACAAATG |
| VlacB | GCATTAGTGTATCAACAAGCTGGGG |
a
Restriction sites are in bold.