TABLE 2.

EC₅₀s of S. gallolyticus subsp. gallolyticus GSP alanine analogs in UCN34 Δgsp^a

Compound	Sequence	EC50 (nM)b	95% CIc
GSP	DFLIVGPFDWLKKNHKPTKHA	2.96	1.70–5.14
GSP D1A	AFLIVGPFDWLKKNHKPTKHA	24.7	12.6–48.3
GSP F2A	DALIVGPFDWLKKNHKPTKHA	19.9	8.79–45.3
GSP L3A	DFAIVGPFDWLKKNHKPTKHA	156	121–200
GSP I4A	DFLAVGPFDWLKKNHKPTKHA	137	61.5–307
GSP V5A	DFLIAGPFDWLKKNHKPTKHA	79.6	51.4–123
GSP G6A	DFLIVAPFDWLKKNHKPTKHA	276	138–553
GSP P7A	DFLIVGAFDWLKKNHKPTKHA	76.5	63.3–92.4
GSP F8A	DFLIVGPADWLKKNHKPTKHA	397	248–635
GSP D9A	DFLIVGPFAWLKKNHKPTKHA	32.6	19.9–53.6
GSP W10A	DFLIVGPFDALKKNHKPTKHA	>1,000
GSP L11A	DFLIVGPFDWAKKNHKPTKHA	718	535–963
GSP K12A	DFLIVGPFDWLAKNHKPTKHA	299	259–346
GSP K13A	DFLIVGPFDWLKANHKPTKHA	297	253–348
GSP N14A	DFLIVGPFDWLKKAHKPTKHA	93.5	61.4–142
GSP H15A	DFLIVGPFDWLKKNAKPTKHA	152	130–177
GSP K16A	DFLIVGPFDWLKKNHAPTKHA	893	515–1,550
GSP P17A	DFLIVGPFDWLKKNHKATKHA	188	89.3–395
GSP T18	DFLIVGPFDWLKKNHKPAKHA	992	722–1,360
GSP K19A	DFLIVGPFDWLKKNHKPTAHA	106	64.4–175
GSP H20A	DFLIVGPFDWLKKNHKPTKAA	273	198–375

^aSee Materials and Methods for details of reporter strains and methods. All assays were performed in triplicate.

^bEC₅₀s were determined by testing peptides over a range of concentrations.

^cCI, confidence interval.