TABLE 3.
EC50s of elongated and truncated S. gallolyticus subsp. gallolyticus GSP analogs in UCN34 Δgspa
| Compound | Sequence | EC50 (nM)b | 95% CIc |
|---|---|---|---|
| KNK-GSP | KNKDFLIVGPFDWLKKNHKPTKHA | 287 | 145–568 |
| NK-GSP | NKDFLIVGPFDWLKKNHKPTKHA | 14.1 | 8.01–24.7 |
| K-GSP | KDFLIVGPFDWLKKNHKPTKHA | 2.33 | 1.45–3.76 |
| GSP-des-D1 | FLIVGPFDWLKKNHKPTKHA | 9.21 | 4.29–19.8 |
| GSP-des-D1-F2 | LIVGPFDWLKKNHKPTKHA | 6.49 | 3.03–13.9 |
| GSP-des-D1-L3 | IVGPFDWLKKNHKPTKHA | 2.22 | 1.41–3.52 |
| GSP-des-D1-I4 | VGPFDWLKKNHKPTKHA | 3.80 | 2.49–5.80 |
| GSP-des-D1-V5 | GPFDWLKKNHKPTKHA | 3.60 | 3.34–3.89 |
| GSP-des-D1-G6 | PFDWLKKNHKPTKHA | 3.19 | 2.54–3.99 |
| GSP-des-D1-P7 | FDWLKKNHKPTKHA | 7.21 | 3.79–13.7 |
| GSP-des-D1-F8 | DWLKKNHKPTKHA | 39.7 | 21.4–73.6 |
| GSP-des-D1-D9 | WLKKNHKPTKHA | 26.9 | 18.3–39.7 |
| GSP-des-D1-W10 | LKKNHKPTKHA | >1,000 | |
| GSP-des-A21 | DFLIVGPFDWLKKNHKPTKH | >1,000 | |
| GSP-des-H20-A21 | DFLIVGPFDWLKKNHKPTK | >1,000 | |
| GSP-des-K19-A21 | DFLIVGPFDWLKKNHKPT | >1,000 |
a
See Materials and Methods for details of reporter strains and methods. All assays were performed in triplicate.
b
EC50s were determined by testing peptides over a range of concentrations.
c
CI, confidence interval.