FIG 10.

Phage RNAs associate strongly with ribosomes. (A and B) Expression and sedimentation of early infection operons 54 and 88 was Northern probed and revealed a strongly ribosome-associated profile. Interestingly, smaller fragments sedimented exclusively before fraction six. (C) In general, phage transcripts sedimented substantially at ribosomal fractions, indicating an efficient transfer to translation or stabilization at the ribosome. Phage tRNAs were also strongly utilized in translation or stabilized at ribosomal subunits, judged by sedimentation in ribosomal fractions.